Exploring the Role of lipin1 in Mitophagy Process Using lipin1 Deficient-EGFP Tagged lC3 Transgenic Mice
Year: 2017
Type: article
Abstract: Exploring the role of lipin1 in mitophagy process using lipin1 deficient-EGFP tagged LC3 transgenic mice Lipin1 (phosphatidic acid phosphatase) is a key molecule in the cells with two functions: first, it converts phosphatidic acid into diacylglycerol in the cytosol which in turn makes triglycerides.Second, in nucleus lipin1 acts as a transcriptional factor which regulates the expression of genes involved in the fatty acid oxidation and lipid metabolism.Clinically, Lpin1 gene mutations have been detected in patients with severe rhabdomyolysis accompanied with aggregated and dysfunctional mitochondria in their type II muscle fiber.Previously, we have observed that mice with lipin1 deficiency had aggregated mitochondria and abnormal autophagosomes formations by electron microscopy.The mechanism underlying the mitochondrial alterations observed in rhabdomyolysis patients is not revealed yet.Therefore, we think there is a link between impaired mitophagy and lipin1 deficiency in skeletal muscle.Mitochondrial quality control is responsible for regulating the turnover of the mitochondria within the cells.Mitochondrial autophagy (mitophagy) is a part of the mitochondrial quality control and considered as a key process within the eukaryotes.Dysregulated mitophagy has been shown to be involved in Parkinson's disease, cardiovascular diseases and neurodegenerative disorders.In this project we want to examine the role of lipin1 deficiency on the impaired mitophagy process in the muscular tissue.We generated a unique mice model called lipin1 -/--EGFP-LC3 transgenic mice to study the mitophagy process.This unique model expresses GFPs tagged to autophagosomes in mitophagy.
Author: Abdullah A. Alshudukhi
Institution: Wright State University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2777998613