Dynamics and mechanisms of intracellular calcium waves elicited by tandem bubble-induced jetting flow
Year: 2017
Type: article
Abstract: Significance Ultrasound-induced microbubble oscillation can lead to cell injury or mechanotransduction via calcium signaling processes such as intracellular calcium waves (ICWs). However, the mechanisms by which microbubbles stimulate ICWs remain unknown. Using a microfluidic system with highly controlled bubble−cell interaction, we identified two distinct types of ICWs: a fast response correlating with significant membrane poration, and a slow response triggered by calcium influx through stretch-activated ion channels. The fast ICWs, distinguished from those under physiological conditions, are associated with cell injuries. We further elicited ICWs without cell injury by displacing integrin-binding beads on the cell membrane under mild cavitation conditions. This study provides mechanistic insights into ICWs for guiding ultrasound therapy in tissue modification, drug delivery, and cell mechanotransduction.
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
Funders: National Science Foundation, National Institutes of Health, Directorate for Engineering, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institute of Biomedical Imaging and Bioengineering, National Center for Research Resources, Division of Civil, Mechanical and Manufacturing Innovation
Awards: G3286690430, Ultra High-Speed Imaging System, G5163442167, Probing the Bioeffects of Cavitation at Single-Cell Level, G6579201575, G6799938735, Innovations in Shock Wave Lithotripsy Technology, Mechanical and Osmotic Signaling in Chondrocytes, Innovations in Shock Wave Lithotripsy Technology
OpenAlex ID: W2778137886