The utility of qNMR to improve accuracy and precision of LC-MS bioanalysis
Year: 2017
Type: article
Abstract: In LC-MS bioanalysis, samples and analytes are quantified against calibration solutions and curves which are derived via serial dilution from stock solutions that are prepared from dry reference standards. The analytical errors associated with the mass and volume measurements required for this preparation of stock solutions can culminate in a variance which might affect bioanalytical data accuracy. Especially in the case of extended studies with intermittent sample analysis, the multiple preparation of separate stock solutions can also adversely affect bioanalytical data precision. Discussed here is an illustrative case study where a single stock solution was utilized for a longitudinal study with multiple data points by means of an orthogonal and synergistic quality control via quantitative NMR methodology resulting in improved bioanalytical data.
Source: Journal of Applied Bioanalysis
DOI: 10.17145/jab.18.005
Authors: Dieter M. Drexler, Colleen A. McNaney, Yingzi Wang, Xiaohua Huang, Michael D. Reily
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $517
OpenAlex ID: W2778362540