Work

Perspectives on the cryoEM 'resolution revolution' from the Protein Data Bank

Year: 2017

Type: article

Abstract: Recent technical advances in electron microscope design, automated cryogenic sample handling, direct electron detection, phase plate optics, and data processing software have ushered in an exciting new era in electron microscopic studies of biological systems.Under favorable circumstances, single-particle methods are yielding near atomic resolution (~2Å) structures of large macromolecular assemblies.Equally exciting are the insights coming from cryo-electron tomography using sub-tomogram averaging and micro-electron diffraction.The impact of these related methodologies and structures therefrom will be discussed from the perspective of the RCSB Protein Data Bank (RCSB PDB; rcsb.org).

Source: Acta Crystallographica Section A Foundations and Advances

DOI: 10.1107/s0108767317097793

Author: Stephen K. Burley

Institution: Rutgers, The State University of New Jersey

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, U.S. Department of Energy, National Institutes of Health, Division of Biological Infrastructure