Exosomal microRNAs derived from colorectal cancer-associated fibroblasts: role in driving cancer progression
Year: 2017
Type: article
Abstract: , we demonstrated that fibroblast exosomes are transferred to colorectal cancer cells, with a resultant increase in cellular microRNA levels, impacting proliferation and chemoresistance. To probe this further, exosomal microRNAs were profiled from paired patient-derived normal and cancer-associated fibroblasts, from an ongoing prospective biomarker study. An exosomal cancer-associated fibroblast signature consisting of microRNAs 329, 181a, 199b, 382, 215 and 21 was identified. Of these, miR-21 had highest abundance and was enriched in exosomes. Orthotopic xenografts established with miR-21-overexpressing fibroblasts and CRC cells led to increased liver metastases compared to those established with control fibroblasts. Our data provide a novel stromal exosome signature in colorectal cancer, which has potential for biomarker validation. Furthermore, we confirmed the importance of stromal miR-21 in colorectal cancer progression using an orthotopic model, and propose that exosomes are a vehicle for miR-21 transfer between stromal fibroblasts and cancer cells.
Source: Aging
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Estimated APC paid: $3,106
Awards: Investigating the trigger for fibroblast senescence in colorectal cancer: a novel role for extracellular vesicles, G41983888, G4307269042, G4386570091, Development of a rapid bacterial identification method based on direct mass spectrometric metabolic profiling, G6114986409, NIHR Senior Investigator Award, G6346276443, G6733929955, G7650111435, G8856394638
OpenAlex ID: W2781198385