Year: 2017
Type: dissertation
Abstract: Maintenance of neural stem cell (NSC) niches is required for the continued growth of the zebrafish retina and forebrain after embryogenesis. The zebrafish mutant kess564, which maps to the ice1 locus, exhibits reduced NSC niches. RNA polymerase II-dependent snRNA transcription requires the little elongation complex (LEC) for which ICE1 is an essential scaffolding component. Mutant NSCs which are normally active no longer express markers of cycling cells and become apoptotic. Furthermore, quiescent NSCs of the mutant retina are unable to dedifferentiate in response to UV lesion. Whole-transcriptome analysis of ice1 mutant larvae show a decrease in CNS and cell cycle levels, and an increase in splicing genes indicative of a possible compensatory mechanism. snRNA transcript levels appear to be unaffected in a subset of NSCs but reduced in differentiated neurons. This work demonstrates that ice1 is essential for NSC maintenance in an in vivo loss of function model.
Source: TSpace (University of Toronto)
Author: Cassandra D’Amata
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2781377360