Year: 2011
Type: dissertation
Abstract: Single cell manipulation techniques have important applications in laboratory and clinical procedures such as intracytoplasmic sperm injection (ICSI) and polar body biopsy\nfor preimplantation genetic diagnosis (PGD). Conventionally, manipulation of cells conducted in these procedures have been performed manually, which entails long training hours and stringent skills. Conventional single cell manipulation also has the limitation of low success rates and poor reproducibility due to human fatigue and skill variations across operators. This research focuses on the integration of computer vision microscopy and control algorithms into a system for the automation of the following single cell manipulation\ntechniques: (1) sperm immobilization, (2) cell aspiration into a micropipette, and cell positioning inside a micropipette, and (3) rotational control of cells in three dimensions. These automated techniques eliminate the need for significant human involvement and long training. Through experimental trials on live cells, the automated techniques demonstrated high success rates.
Source: TSpace (University of Toronto)
Author: Clement H. C. Leung
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Funder: University of Toronto
OpenAlex ID: W2782346413