Work

Premutilin Synthase: Ring Rearrangement by a Class II Diterpene Cyclase

Year: 2018

Type: article

Abstract: Biosynthesis of the complex diterpenoid antibiotic pleuromutilin relies on a bifunctional (di)terpene synthase, and here site-directed mutagenesis was used to knockout either of the two active sites. This enabled characterization of the novel ring contracted intermediate produced by the initiating class II diterpene cyclase active site. Quantum chemical calculations further indicate the importance of reactant configuration for this intriguing ring rearrangement.

Source: Organic Letters

DOI: 10.1021/acs.orglett.8b00121

Authors: Meimei Xu, Meirong Jia, Young J. Hong, Xihou Yin, Dean J. Tantillo, Philip J. Proteau, Reuben J. Peters

Institutions: Iowa State University, University of California, Davis, Oregon State University

Language: English

Topic: Plant biochemistry and biosynthesis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, Division of Chemistry

Awards: G2508209878, G5280846968, G6588461257, Biosynthesis of (di)terpene scaffolds, G8326735537

OpenAlex ID: W2785419664

FWCI: 1.245

Cites: 23

Cited by: 30

Related to: 10