Work

Development of a universal, tunable, miniature fluorescence microscope for use at the point of care

Year: 2018

Type: article

Abstract: Fluorescence microscopy can be a powerful tool for cell-based diagnostic assays; however, imaging can be time consuming and labor intensive to perform. Tunable systems give the ability to electronically focus at user selected depths inside an object volume and may simplify the opto-mechanical design of the imaging system. We present a prototype of a universal, tunable, miniature fluorescence microscope built from poly(methyl methacrylate) singlets that incorporates miniature, electrowetted lenses for electronic focusing. We demonstrate the ability of this system to perform clinically relevant differential white blood cell counts using single use custom cartridges pre-loaded with the fluorescent dye acridine orange.

Source: Biomedical Optics Express

DOI: 10.1364/boe.9.001041

Authors: Cynthia Wong, Michal E. Pawlowski, Alessandra Forcucci, Catherine E. Majors, Rebecca Richards-Kortum, Tomasz S. Tkaczyk

Institution: Rice University

Language: English

Topic: Advanced optical system design

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: Doris Duke Charitable Foundation, National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering