Fibrinogen and mucin binding activity of EF0737, a novel protein of Enterococcus faecalis.
Year: 2017
Type: article
Abstract: BACKGROUND AND OBJECTIVES: , based on our experiments. This study was conducted to clone and express EF0737 and demonstrate its interaction with biotinylated plasma proteins and patients' sera. MATERIALS AND METHODS: Origami (DE3) was confirmed by western blot technique, using anti-His tagged monoclonal antibodies, and was then purified. Interaction of the recombinant protein with plasma proteins and patients' sera were examined by western blot. RESULTS: infection. CONCLUSION: . However, more comprehensive characterization of this novel protein may provide better understanding of host pathogen interaction.
Source: PubMed Central
Institution: Tehran University of Medical Sciences
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2787331246