Work

Post-transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs

Year: 2011

Type: book-chapter

Abstract: RNA-guided RNA 2'-O-methylation and pseudouridylation are naturally occurring processes, in which guide RNAs specifically direct modifications to rRNAs or spliceosomal snRNAs in the nucleus of eukaryotic cells. Modifications can profoundly alter the properties of an RNA, thus influencing the contributions of the RNA to the cellular process in which it participates. Recently, it has been shown that, by expressing artificial guide RNAs (derived from naturally occurring guide RNAs), modifications can also be specifically introduced into other RNAs, thus offering an opportunity to study RNAs in vivo. Here, we present strategies for constructing guide RNAs and manipulating RNA modifications in the nucleus.

Source: Methods in molecular biology

DOI: 10.1007/978-1-61779-018-8_14

Authors: Chao Huang, John Karijolich, Yi-Tao Yu

Institutions: University of Rochester Medicine, University of Rochester

Language: English

Topic: RNA modifications and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: G2100436727, Spliceosomal snRNA modification in Xenopus oocytes, G508708373, Training in Cellular, Biochemical and Molecular Sciences