A Unique Gene Expression Profile to Identify Operationally Tolerant Liver Transplant Recipients
Year: 2016
Type: dissertation
Study design: Observational Study
Abstract: Identification of operationally tolerant liver transplant recipients (TOL) would allow for withdrawal of immunosuppression and potential improvement in long-term graft survival. We investigated the utility of a previously identified pre-clinical gene expression profile to identify TOL. Peripheral blood mononuclear cells (PBMC) were isolated from TOL (N= 4), rejecting liver transplant recipients (REJ, N= 6); liver transplant recipients on minimal immunosuppression (Min IS, N= 29),on standard immunosuppression (Stand IS, N= 24), and healthy live liver donors (HLD, N= 23). Liver biopsies and plasma cytokine levels were also obtained from a subset of patients to compare intra-graft gene expression with gene expression and protein levels in PBMC. All 4 liver transplant recipients who had developed a state of tolerance and were off immunosuppression (TOL) had high co-expression of FGL2 and TIGIT mRNA transcripts in their PBMC in contrast to patients undergoing rejection yielding a potentially useful biomarker panel for operational tolerance.
Source: TSpace (University of Toronto)
Author: Vanessa Rojas Luengas
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2789466283