Work

Structures and disulfide cross‐linking of de novo designed therapeutic mini‐proteins

Year: 2018

Type: article

Abstract: Recent advances in computational protein design now enable the massively parallel de novo design and experimental characterization of small hyperstable binding proteins with potential therapeutic activity. By providing experimental feedback on tens of thousands of designed proteins, the design-build-test-learn pipeline provides a unique opportunity to systematically improve our understanding of protein folding and binding. Here, we review the structures of mini-protein binders in complex with Influenza hemagglutinin and Bot toxin, and illustrate in the case of disulfide bond placement how analysis of the large datasets of computational models and experimental data can be used to identify determinants of folding and binding.

Source: FEBS Journal

DOI: 10.1111/febs.14394

Authors: Daniel‐Adriano Silva, Lance Stewart, Kwok‐Ho Lam, Rongsheng Jin, David Baker

Institutions: University of Washington, University of California, Irvine

Language: English

Topic: Protein Structure and Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $5,000

Funders: Washington Research Foundation, Consejo Nacional de Ciencia y Tecnología, National Institutes of Health, National Institute of Allergy and Infectious Diseases