Year: 2018
Type: preprint
Abstract: Cardiovascular lineages develop together with kidney, smooth muscle, and limb connective tissue progenitors from the lateral plate mesoderm (LPM). How the LPM initially emerges and how its downstream fates are molecularly interconnected remain unknown. Here, we isolated a pan-LPM enhancer in the zebrafish draculin ( drl ) gene that provides specific LPM reporter activity from early gastrulation. In toto live imaging and lineage tracing of drl -based reporters captured the dynamic LPM emergence as lineage-restricted mesendoderm field. The drl pan-LPM enhancer responds to the transcription factors EomesoderminA, FoxH1, and MixL1 that combined with Smad activity drive LPM emergence. We uncovered specific drl reporter activity in LPM-corresponding territories of several chordates including chicken, axolotl, lamprey, Ciona , and amphioxus, revealing a universal upstream LPM program. Altogether, our work provides a mechanistic framework for LPM emergence as defined progenitor field, possibly representing an ancient mesodermal cell state that predates the primordial vertebrate embryo.
DOI: 10.1101/261115
Authors: Karin D. Prummel, Christopher Hess, Susan Nieuwenhuize, Hugo J. Parker, Katherine W. Rogers, Iryna Kozmikova, Claudia Racioppi, Eline C. Brombacher, Anna Czarkwiani, Dunja Knapp, Sibylle Burger, Elena Chiavacci, Gopi Shah, Alexa Burger, Jan Huisken, Maximina H. Yun, Lionel Christiaen, Zbynek Kozmik, Patrick Müller, Marianne Bronner, Robb Krumlauf, Christian Mosimann
Institutions: University of Zurich, Stowers Institute for Medical Research, University of Kansas Medical Center, Max Planck Society, Friedrich Miescher Laboratory, Czech Academy of Sciences, Institute of Molecular Genetics, New York University, Center for Regenerative Therapies Dresden, Technische Universität Dresden, Max Planck Institute of Molecular Cell Biology and Genetics, Morgridge Institute for Research, California Institute of Technology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: National Science Foundation, Company of Biologists, European Commission, Deutsche Forschungsgemeinschaft, Fondation Leducq, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Schweizerische Herzstiftung, UZH Foundation, National Institutes of Health, Foundation for Research in Science and the Humanities, National Heart, Lung, and Blood Institute
Awards: G2114980211, 22q11.2 deletion syndrome: Novel approaches to understand cardiopharyngeal pathogenesis, G2462971813, G2551189203, Cellular determinants of cardiopharyngeal multipotency and early fate choices, Lightsheet Fluorescence Microscopy, Mechanisms of earliest hematopoietic cell fate control, G62859826, G640430542, G7089742587, G7186293720, Deciphering the control of lateral mesoderm cell fates in zebrafish
OpenAlex ID: W2790991082