Year: 2017
Type: article
Abstract: Irradiation of red blood cells prior to transfusion is required to prevent transfusion associated graft versus host disease for patients undergoing hematopoietic stem cell transplant.Additional application for irradiated blood may exist in oncological surgery.The effect of irradiation on canine packed red blood cells (pRBCs) is unknown.The aim of this study was to explore and characterize the in vitro lymphocyte viability, lymphocyte activation potential, electrolyte, acid-base and oxygen carrying capacity changes to pRBCs immediately following irradiation and with storage.Ten units of pRBCs were irradiated using a linear accelerator.Flow cytometric analysis of percentage viability, percentage of CD3 and CD25 expression and mean fluorescence intensity (MFI) of CD25 was performed immediately following irradiation, and at days 7, 10 and 17 of storage.Potassium, glucose, percentage of free hemoglobin (fHb), p50, hemoglobin oxygen saturation (sO2), total oxygen content, partial pressure of oxygen (pO2), lactate, pH, methemoglobin, white cell count (WCC) and lymphocyte count were measured before and immediately following irradiation, and 7 and 17 days post-irradiation.Significant differences were observed in lymphocyte viability following irradiation.However, these changes were not noted immediately following irradiation, but rather were noted following incubation or storage.Irradiation induced activation in lymphocytes as indicated by an increase in the percentage of viable lymphocytes iii expressing both CD3 and CD25 in irradiated cells at day 0 following incubation.A significant decrease in the percentage of viable lymphocytes over time was noted in both irradiated and unirradiated cells, similar to changes found in the WCC over time.Significant changes were noted in pH, potassium, glucose, lactate, fHb, pO2 and the p50 value in both irradiated and unirradiated units over time.Immediately following irradiation, pH, potassium, lactate and fHb were significantly different between irradiated units and matched controls.Relatively small but significant differences were also noted between irradiation status in pH, fHb, sO2, total oxygen content and p50 value 7 days post-irradiation.Only sO2 differed at day 17.We found that irradiation and storage altered in vitro properties of pRBCs compared to unirradiated blood.Because our results using viability assessment did not demonstrate complete loss of lymphocytes following irradiation, further work may be required to validate this irradiation protocol in canine blood using more sensitive lymphocyte proliferation indices.The finding of highly activated lymphocytes following irradiation also warrants further investigation, including the clinical consequences of transfusing these cells.The magnitude of the differences in electrolyte, acid-base and oxygen carrying capacity was small.The clinical impact of irradiation on these variables may be negligible and unlikely to preclude safe use of these products in clinical patients.Eric Green for their assistance and support in the completion of this project.Including Dr.Cooper, my residency mentors Drs.Page Yaxley and Julien Guillaumin also deserve a special mention, as the training and guidance through my residency has allowed me to develop into better doctor, teacher and researcher.I would like to express heartfelt thanks to Dr. Claire Fellman, whose tremendous knowledge, training and expertise allowed this project to be possible, and whose endless patience as I learnt the ropes of flow cytometry was invaluable during the genesis and execution of this project.
Author: Saya A. Press
Institution: The Ohio State University
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2792269876