Year: 2018
Type: data-paper
Abstract: Although hair cells are the sensory receptors of the auditory and vestibular systems in the ears of all vertebrates, hair cell properties are different between non-mammalian vertebrates and mammals. To understand the basic biological properties of hair cells from non-mammalian vertebrates, we examined the transcriptome of adult zebrafish auditory and vestibular hair cells. GFP-labeled hair cells were isolated from inner-ear sensory epithelia of a pou4f3 promoter-driven GAP-GFP line of transgenic zebrafish. One thousand hair cells and 1,000 non-sensory surrounding cells (nsSCs) were separately collected for each biological replicate, using the suction pipette technique. RNA sequencing of three biological replicates for the two cell types was performed and analyzed. Comparisons between hair cells and nsSCs allow identification of enriched genes in hair cells, which may underlie hair cell specialization. Our dataset provides an extensive resource for understanding the molecular mechanisms underlying morphology, function, and pathology of adult zebrafish hair cells. It also establishes a framework for future characterization of genes expressed in hair cells and the study of hair cell evolution.
Source: Scientific Data
DOI: 10.1038/sdata.2018.5
Language: English
Subfield: Sensory Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,490
Awards: Nebraska Research Network in Functional Genomics, G1934757924, The Molecular Biology of Neurosurgery Systems, Biophysics and Development of Cochlear Hair Cells, G337890086, The Molecular Biology of Neurosurgery Systems, G5318962234, UNMC SIG Supporting Next-generation DNA Sequencing Technology, Nebraska Research Network in Functional Genomics, G7708809446, The Molecular Biology of Neurosensory Systems, G839628966, G8825797209
OpenAlex ID: W2792770106