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The effects of aging on biosynthetic processes in the rat hypothalamic osmoregulatory neuroendocrine system

Year: 2018
Type: article
Abstract: Elderly people exhibit a diminished capacity to cope with osmotic challenges such as dehydration. We have undertaken a detailed molecular analysis of arginine vasopressin (AVP) biosynthetic processes in the supraoptic nucleus (SON) of the hypothalamus and secretory activity in the posterior pituitary of adult (3 months) and aged (18 months) rats, to provide a comprehensive analysis of age-associated changes to the AVP system. By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis, we identified differences in pituitary peptides, including AVP, in adult and aged rats under both basal and dehydrated states. In the SON, increased Avp gene transcription, coincided with reduced Avp promoter methylation in aged rats. Based on transcriptome data, we have previously characterized a number of novel dehydration-induced regulatory factors involved in the response of the SON to osmotic cues. We found that some of these increase in expression with age, while dehydration-induced expression of these genes in the SON was attenuated in aged rats. In summary, we show that aging alters the rat AVP system at the genome, transcriptome, and peptidome levels. These alterations however did not affect circulating levels of AVP in basal or dehydrated states.
DOI: 10.1016/j.neurobiolaging.2018.01.008
Authors: Michael P. Greenwood, Mingkwan Greenwood, Elena V. Romanova, Andre S. Mecawi, Alex Paterson, Olivera Sarenac, Nina Japundžić-Žigon, José Antunes-Rodrigues, Julian F.R. Paton, Jonathan V. Sweedler, David Murphy
Institutions: University of Bristol, University of Illinois Urbana-Champaign, Universidade Federal Rural do Rio de Janeiro, Universidade de São Paulo, University of Malaya, University of Belgrade
Language: English
Field: Psychology
Open Access status: hybrid
Estimated APC paid: $3,430
Funders: British Heart Foundation, Fundação de Amparo à Pesquisa do Estado de São Paulo, Max-Planck-Gesellschaft, Universiti Malaya, National Institutes of Health, Medical Research Council, Biotechnology and Biological Sciences Research Council, National Institute on Drug Abuse
Awards: G1647085368, G1806490181, Gene networks involved in hypothalamic plasticity in response to dehydration; assessing the in vivo functions of candidate nodal genes., G2586551248, G3060884880, G3144748132, G3483451039, USA - Novel insights into the mechanisms of salt appetite, Brainstem hypoperfusion as a causative mechanism for neurogenic hypertension (renewal), Bilateral BBSRC-FAPESP: Behavioural and neuroendocrine mechanisms regulating hydromineral homeostasis - a lifelong perspective, Energy balance and body fluid homeostasis control: from cells to the physiological systems, G600732768, Regulatory and functional pathways mediating the control of central osmotic defences by hypothalamic transcription factor CREB3L1, G9000216650
OpenAlex ID: W2793200550
FWCI: 2.887
Cites: 74
Cited by: 23
Related to: 10