Work

Precision Tagging: A Novel Seamless Protein Tagging by Combinational Use of Type II and Type IIS Restriction Endonucleases

Year: 2018

Type: article

Abstract: , 2017), which generates a seamless clone with high efficiency. Here, we describe a protocol that not only provides a generalized strategy for any gene of interest but also takes its application of 11 different tags in THSD1 as a step-by-step example.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.2721

Authors: Zhen Xu, Yan-Ning Rui, John Hagan, Dong Kim

Institution: The University of Texas Health Science Center at Houston

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Neurological Disorders and Stroke

Award: G5421574199