Work

FRET-based Stoichiometry Measurements of Protein Complexes in vitro

Year: 2018

Type: article

Abstract: . This assay, also known as Job plot, is set up as a continuous variation of the molar ratio between the two species, kept at constant total concentration. The FRET (Fluorescence Resonance Energy Transfer) between the two fluorescently-labeled proteins is measured and the stoichiometry is inferred from the sample with highest FRET signal. This approach allows us to assess complex stoichiometry in solution.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.2713

Authors: Francesca Mattiroli, Yajie Gu, Karolin Luger

Institutions: Howard Hughes Medical Institute, University of Colorado Boulder, Colorado State University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: G7474329723