Year: 2018
Type: article
Abstract: Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging and produce unwanted side effects. Here we develop and validate a nanodelivery approach to transfer in vitro synthesized, functional membrane receptors into the plasma membrane of living cells. Using β 2 -adrenergic receptor (β 2 AR), a prototypical G-protein coupled receptor, as an example, we demonstrated efficient incorporation of a full-length β 2 AR into a variety of mammalian cells, which imparts pharmacologic control over cellular signaling and affects cellular phenotype in an ex-vivo wound-healing model. Our approach for nanodelivery of functional membrane receptors expands the current toolkit for DNA and RNA-free manipulation of cellular function. We expect this approach to be readily applicable to the synthesis and nanodelivery of other types of GPCRs and membrane receptors, opening new doors for therapeutic development at the intersection between synthetic biology and nanomedicine.
Source: Scientific Reports
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,690
Funders: U.S. Department of Energy, U.S. Department of Veterans Affairs, American Heart Association, National Institutes of Health, National Institute of Food and Agriculture, University of California, Davis, National Heart, Lung, and Blood Institute, National Institute of Mental Health, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering, Lawrence Livermore National Laboratory
Awards: G1356433355, G14554683, G1716777315, G2145270357, The rodent eye as a non-invasive window for understanding cancer nanotherapeutics, Genetically Encoded Small Illuminants for 4D nucleome imaging, Fluorescent biosensors for imaging neurotransmitters: observing synapses in actio, G3669113783, Characterization of Erbb Receptors in Nanoparticles, G4343739756, Genetically encoded sensors for the biogenic amines: watching neuromodulation in action, Insulin Inhibition of beta-AR Signaling in the Myocardium, G69709960, Present Homologous and Heterologous Antigen with Hepatitis E Virus, G7737658675, G7790607044, Cardiac Dysfunction in the Met Syndrome: Cross-talk between IR and bAR Signaling, G895790057, G9000240216, G991281326
OpenAlex ID: W2794045875