Work

FACS-based Glucose Uptake Assay of Mouse Embryonic Fibroblasts and Breast Cancer Cells Using 2-NBDG Probe

Year: 2018

Type: article

Abstract: , 2017 ). Briefly, the target cells are incubated with the fluorescently tagged 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) for 2 h or 30 min, and the efficiency of glucose uptake is examined using a flow cytometer. This method can be adapted to measure a variety of adipocytes, immune cells, MEFs and cancer cells.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.2816

Authors: Shengli Dong, Suresh Alahari

Institutions: Louisiana State University Health Sciences Center New Orleans, Louisiana State University

Language: English

Topic: Metabolism, Diabetes, and Cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2794378289

FWCI: 0.089

Cites: 10

Cited by: 10

Related to: 10