Chenodeoxycholic acid improves intestinal permeability in piglets
Year: 2012
Type: conference-abstract
Abstract: Piglets are highly susceptible to gut health-related problems. Intravenously administered chenodeoxycholic acid (CDCA) affects gut health mediated through GLP-2. To test if CDCA is a suitable feed additive for improving gut health, a trial was performed with newly weaned (21 d) piglets offered a diet with or without 60 mg CDCA/kg feed (n = 24/trt). Upon weaning piglets were fasted for 16 h and then intragastrically dosed with 20 g test feed in 40 g water. Subsequently, a jugular blood sample was taken on either 45, 90, 135, or 180 min for analysis of GLP-2, PYY, and glucose. Afterward, piglets were fed ad libitum. On d 3.5, 7.5, and 10.5 post weaning, 8 piglets per treatment were sacrificed for determination of in vivo intestinal permeability using lactulose and Co-EDTA. Both markers were administrated intragastrically and after 2h, a blood sample was obtained through venipuncture. Immediately thereafter, intestines were harvested, and ex vivo permeability was measured using the everted gut sac technique with 4 kDa FITC-dextran as marker at 25, 50, and 75% of the length of the small intestines. Average daily feed intake, daily gain, gain:feed, blood glucose, plasma GLP-2, and PYY were not affected by dietary CDCA (P > 0.10). Serum Co-EDTA and lactulose concentrations at d 10.5 tended to be lower in CDCA pigs compared with the control pigs (P = 0.054, P = 0.089). The everted gut sac technique data did not show any treatment effects on permeability (P > 0.10), possibly due to reaction of the FITC-marker with light despite covering with aluminum foil. In conclusion, CDCA tended to improve intestinal permeability at 10.5 d post weaning when fed to newly weaned piglets, implying that CDCA deserves further study as a means for improving intestinal health
Authors: Y. van der Meer, W.J.J. Gerrits, M. van den Bosch, Jens J. Holst, Wim Kulik, T. A. T. G. van Kempen
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2795166914