Mp29-08 Hsd3b1 Preferentially Utilizes Pregnenolone to Promote Resistance to Anti-androgens in Prostate Cancer
Year: 2018
Type: article
Abstract: prostatectomy (RALP).In vitro characterization included proliferation assay and the expression of potential markers of carcinoma associated fibroblasts (CAF).The effects of AA vs. CAU fibroblasts on prostate cancer cell proliferation and motility were studied.In vivo pro-tumorigenic properties of fibroblasts were tested using a tissue recombination and subrenal xenograft model in SCID mice.Cytokine array analysis of potential paracrine mediators of tumorigenesis was performed RESULTS: Prostate fibroblasts from AA showed increased proliferation response to mitogens compared to CAU.Expression of markers associated with myofibroblast activation (aSMA, vimentin and Tenascin-C) as well as AR were significantly elevated in AA, while Caveolin 1 expression was lower in AA fibroblasts relative to CAU.AA fibroblasts significantly increased the tumorigenicity in vitro and in vivo of an AA patient-derived prostatic epithelial cell line E006AA compared to CAU.Analysis of potential paracrine mediators identified a panel of pro-inflammatory cytokines notably interleukins (IL6, IL11, IL17, IL18BP), growth factors (VEGF, FGF, BDNF), and other mediators to be enriched in AA fibroblasts CONCLUSIONS: Prostate fibroblasts from African American men show enhanced secretion of pro-inflammatory mediators that can potentially increase the tumorigenicity of PCa cells through selective paracrine mechanisms
Source: The Journal of Urology
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2796025636