MP54-06 THREE-DIMENSIONAL ORGANOID CULTURE REVEALS INVOLVEMENT OF WNT/β-CATENIN PATHWAY IN PROLIFERATION OF BLADDER CANCER CELLS
Year: 2018
Type: article
Abstract: IHC) on tissue microarrays were used to confirm tissue sampling and the gene expression analysis.RESULTS: Unsupervised consensus clustering yielded four distinct consensus clusters (CC) or subtypes: CC1-Basal, CC2-Luminal, CC3-Immune and CC4-Scar-like.The CC1-Basal and CC2-Luminal subtypes expressed genes consistent with a basal-like (KRT5/ 6, KRT14) and a luminal-like (GATA3, PPARG) phenotype, respectively.The CC3-Immune subtype had the highest immune activity, including T-cell infiltration and checkpoint molecules (CTLA4, CD80).Finally, the CC4-Scar-like profile was consistent with non-neoplastic scar samples, expressing genes associated with wound healing / scarring (MYH11, SHH, CNN1).Approximately the same numbers of pre-NAC basal-and luminal-like samples remained static (CC1 or CC2) with respect to subtype or were highly immune-infiltrated (CC3) in the post-NAC setting.In the post-NAC setting, luminal-like pre-NAC samples were more likely adopt a scar-like character (CC4).CONCLUSIONS: This study expands our knowledge of cisplatin-resistant MIBC by suggesting molecular subtypes to understand the biology of these tumors.Although these molecular subtypes imply consequences for adjuvant treatments, this ultimately needs to be tested in clinical trials.
Source: The Journal of Urology
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2796132972