Work

Effects of 7-MEGATM500 on Oxidative Stress, Inflammation, and Skin Regeneration in H2O2-Treated Skin Cells

Year: 2018

Type: article

Abstract: Environmental stimuli can lead to the excessive accumulation of reactive oxygen species (ROS), which is one of the risk factors for premature skin aging.Here, we investigated the protective effects of 7-MEGA TM 500 (50% palmitoleic acid, 7-MEGA) against oxidative stress-induced cellular damage and its underlying therapeutic mechanisms in the HaCaT human skin keratinocyte cell line (HaCaT cells).Our results showed that treatment with 7-MEGA prior to hydrogen peroxide (H 2 O 2 )-induced damage significantly increased the viability of HaCaT cells.7-MEGA effectively attenuated generation of H 2 O 2 -induced reactive oxygen species (ROS), and inhibited H 2 O 2induced inflammatory factors, such as prostaglandin E 2 (PGE 2 ), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β).In addition, cells treated with 7-MEGA exhibited significantly decreased expression of matrix metalloproteinase-1 (MMP-1) and increased expression of procollagen type 1 (PCOL1) and Elastin against oxidative stress by H 2 O 2 .Interestingly, these protective activities of 7-MEGA were similar in scope and of a higher magnitude than those seen with 98.5% palmitoleic acid (PA) obtained from Sigma when given at the same concentration (100 nL/mL).According to our data, 7-MEGA is able to protect HaCaT cells from H 2 O 2 -induced damage through inhibiting cellular oxidative stress and inflammation.Moreover, 7-MEGA may affect skin elasticity maintenance and improve skin wrinkles.These findings indicate that 7-MEGA may be useful as a food supplement for skin health.

Source: Toxicological Research

DOI: 10.5487/tr.2018.34.2.103