Year: 2018
Type: preprint
Abstract: EquCab2, a high-quality reference genome for the domestic horse, was released in 2007. Since then, it has served as the foundation for nearly all genomic work done in equids. Recent advances in genomic sequencing technology and computational assembly methods have allowed scientists to improve reference assemblies of large animal and plant genomes in terms of contiguity and composition. In 2014, the equine genomics research community began a project to improve the reference sequence for the horse, building upon the solid foundation of EquCab2 and incorporating new short-read data, long-read data, and proximity ligation data. The result, EquCab3, is presented here. The count of non-N bases in the incorporated chromosomes is improved from 2.33Gb in EquCab2 to 2.41Gb from EquCab3. Contiguity has also been improved nearly 40-fold with a contig N50 of 4.5Mb and scaffold contiguity enhanced to where all but one of the 32 chromosomes is comprised of a single scaffold.
DOI: 10.1101/306928
Authors: Theodore S. Kalbfleisch, Edward S. Rice, Michael S. DePriest, Brian P. Walenz, Matthew S. Hestand, Joris R. Vermeesch, Brendan L. O’Connell, Ian T. Fiddes, Alisa O. Vershinina, Jessica L. Petersen, Carrie J. Finno, Rebecca R. Bellone, Molly E. McCue, Samantha A. Brooks, Ernest Bailey, Ludovic Orlando, Richard E. Green, Donald C. Miller, Douglas F. Antczak, James N. MacLeod
Institutions: University of Louisville, University of California, Santa Cruz, National Institutes of Health, National Human Genome Research Institute, KU Leuven, University of Nebraska–Lincoln, University of California, Davis, University of Minnesota, University of Florida, University of Kentucky, Centre National de la Recherche Scientifique, Université Toulouse III - Paul Sabatier, AMIS - Laboratoire d'anthropologie moléculaire et imagerie de synthèse, Natural History Museum of Denmark, Centre d'Anthropobiologie et de Génomique de Toulouse, Université de Toulouse, Cornell University, New York State College of Veterinary Medicine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2798866848