Work

Improvement of Lotus japonicus hairy root induction and development of a mycorrhizal symbiosis system

Year: 2018

Type: article

Abstract: Premise of the Study We describe a highly efficient in vitro Lotus japonicus hairy root transformation system that is useful for the investigation of mycorrhizal symbiosis. Methods and Results The Agrobacterium rhizogenes–mediated transformation method was improved based on the binary plasmid (pCAMBIA1304) harboring green fluorescent protein and β‐glucuronidase genes for rapid detection. Transgenic hairy roots were grown within 13 days. These in vitro–cultured hairy roots can be inoculated with Rhizophagus irregularis, thus facilitating the investigation of the symbiosis between L. japonicus and arbuscular mycorrhizal fungi. Conclusions Compared with existing techniques, our protocol provides a simple and efficient A. rhizogenes–mediated transformation system for L. japonicus. The rapid induction of hairy roots can shorten the experimental time by at least one week.

Source: Applications in Plant Sciences

DOI: 10.1002/aps3.1141

Authors: Yunjian Xu, Fang Liu, Guomin Han, Wei Wang, Suwen Zhu, Xiaoyu Li

Institution: Anhui Agricultural University

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $2,340