Sample manipulation and data assembly for robust microcrystal synchrotron crystallography

Year: 2018

Type: article

Abstract: With the recent developments in microcrystal handling, synchrotron microdiffraction beamline instrumentation and data analysis, microcrystal crystallo-graphy with crystal sizes of less than 10 µm is appealing at synchrotrons. However, challenges remain in sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Here, the development of micro-sized polyimide well-mounts for the manipulation of microcrystals of a few micrometres in size and the implementation of a robust data-analysis method for the assembly of rotational microdiffraction data sets from many microcrystals are described. The method demonstrates that microcrystals may be routinely utilized for the acquisition and assembly of complete data sets from synchrotron microdiffraction beamlines.

Source: IUCrJ

DOI: 10.1107/s2052252518005389

Authors: Gongrui Guo, Martin R. Fuchs, Wuxian Shi, John Skinner, Evanna Berman, Craig M. Ogata, Wayne A. Hendrickson, Sean McSweeney, Qun Liu

Institutions: Brookhaven National Laboratory, National Synchrotron Light Source II, Argonne National Laboratory, Advanced Photon Source, Columbia University

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,200

Funders: Foundation for the National Institutes of Health, U.S. Department of Energy, Savannah River Operations Office, U.S. Department of Energy, National Institutes of Health, Office of Science, National Institute of General Medical Sciences, National Institute of Biomedical Imaging and Bioengineering, Basic Energy Sciences, Biological and Environmental Research, Laboratory Directed Research and Development, Brookhaven National Laboratory

OpenAlex ID: W2799524751