Year: 2018
Type: letter
Abstract: Our group reported the first reversible reaction-based ratiometric probe—ThiolQuant Green—that can quantify glutathione (GSH) levels in living cells in 2015 1 . Building on this work, we developed the second generation probe, RealThiol (RT), which has a much improved reaction kinetics, quantum yield, and solubility, enabling quantitative real-time monitoring of GSH level changes in living cells 2 . Recently, we developed a mitochondria-specific GSH probe, MitoRT, which can monitor mitochondrial GSH (mGSH) dynamics 3 . The RT and MitoRT probes have generated tremendous interest in the redox biology community and been shared with more than 40 laboratories around the globe through either collaboration or kerafast.com. As one of the early adopters, the Schmidt group applied RT to monitor GSH level changes in sulfur mustard resistant keratinocytes 4 . Despite of the success of our GSH probes, each method has its own pros and cons. We appreciate Cossetti et al.’s effort to validate the RT probe and agree with their main conclusion that RT can be used as a GSH probe only when the amount of GSH is at least an order of magnitude greater than that of cysteine (Cys). Taking advantage of this opportunity, we will emphasize the limitations of the RT probe and clarify the seemingly discrepancies in Cossetti et al.’s correspondence.
Source: Nature Communications
Authors: Xiqian Jiang, Jianwei Chen, Jin Wang
Institution: Baylor College of Medicine
Language: English
Topic: Sulfur Compounds in Biology
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
OpenAlex ID: W2800076457