Plasma and memory B cell responses targeting O-specific polysaccharide (OSP) are associated with protection against Vibrio cholerae O1 infection among household contacts of cholera patients in Bangladesh
Year: 2018
Type: article
Study design: Observational Study
Abstract: BACKGROUND: The mediators of protection against cholera, a severe dehydrating illness of humans caused by Vibrio cholerae, are unknown. We have previously shown that plasma IgA as well as memory B IgG cells targeting lipopolysaccharide (LPS) of Vibrio cholerae O1 correlate with protection against V. cholerae O1 infection among household contacts of cholera patients. Protection against cholera is serogroup specific, and serogroup specificity is defined by the O-specific polysaccharide (OSP) component of LPS. Therefore, we prospectively followed household contacts of cholera patients to determine whether OSP-specific immune responses present at the time of enrollment are associated with protection against V. cholerae infection. METHODOLOGY: In this study, we enrolled two hundred forty two household contacts of one hundred fifty index patients who were infected with Vibrio cholerae. We determined OSP-specific memory B cells and plasma IgA, IgG and IgM antibody responses on study entry (day 2). PRINCIPLE FINDINGS: The presence of OSP-specific plasma IgA, IgM, and IgG antibody responses on study entry were associated with a decrease in the risk of infection in household contacts (IgA, p = 0.015; IgM, p = 0.01, and IgG, p = 0.024). In addition, the presence of OSP-specific IgG memory B cell responses in peripheral blood on study entry was also associated with a decreased risk of infection (44% reduction; 95% CI: 31.1 to 99.8) in contacts. No protection was associated with cholera toxin B subunit (CtxB)-specific memory B cell responses. CONCLUSION: These results suggest that immune responses that target OSP, both in plasma and memory responses, may be important in mediating protection against infection with V. cholerae O1.
Source: PLoS neglected tropical diseases
Authors: Amena Aktar, M. Arifur Rahman, Sadia Afrin, Aklima Akter, Taher Uddin, Tahirah Yasmin, Md. Israk Nur Sami, Pinki Dash, Sultana Rownok Jahan, Fahima Chowdhury, Ashraful I. Khan, Regina C. LaRocque, Richelle C. Charles, Taufiqur Rahman Bhuiyan, Anjali Mandlik, Meagan Kelly, Pavol Kováč, Peng Xu, Stephen B. Calderwood, Jason B. Harris, Firdausi Qadri, Edward T. Ryan
Language: English
Subfield: Endocrinology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,670
Funders: Howard Hughes Medical Institute, Robert Wood Johnson Foundation, Styrelsen för Internationellt Utvecklingssamarbete, International Centre for Diarrhoeal Disease Research, Bangladesh, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Fogarty International Center, National Institute of Diabetes and Digestive and Kidney Diseases
Awards: Protecutive immunity to human cholera in Bangladesh, G1313941599, G1510978416, G1873111418, O-Specific Polysaccharide Responses and Cholera, G3472663962, G3674028537, G3769145211, High-throughput proteomic study of protective immunity in human cholera infection, G516757433, G5551172042, G6010259269, Mucosal immune response to oral cholera vaccine Peru 15, Innate and early B cell responses to V. cholerae, G7878150564, Assessing measures to eliminate transmission of cholera in Haiti, Humoral and adaptive immune responses study in Vibrio cholerae infection, G9000044515, O-Specific Polysaccharide Responses and Cholera, Center for the Study of Inflammatory Bowel Disease at Massachusetts General Hospital, International research training program in vaccine development and public health
OpenAlex ID: W2800601437