Work

Lifting degeneracy in holographic characterization of colloidal particles using multi-color imaging

Year: 2018

Type: article

Abstract: Micrometer sized particles can be accurately characterized using holographic video microscopy and Lorenz-Mie fitting. In this work, we explore some of the limitations in holographic microscopy and introduce methods for increasing the accuracy of this technique with the use of multiple wavelengths of laser illumination. Large high index particle holograms have near degenerate solutions that can confuse standard fitting algorithms. Using a model based on diffraction from a phase disk, we explain the source of these degeneracies. We introduce multiple color holography as an effective approach to distinguish between degenerate solutions and provide improved accuracy for the holographic analysis of sub-visible colloidal particles.

Source: Optics Express

DOI: 10.1364/oe.26.013239

Authors: David B. Ruffner, Fook Chiong Cheong, Jaroslaw M. Blusewicz, Laura A. Philips

Institution: Spheryx (United States)

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funders: National Science Foundation, National Institutes of Health, National Center for Advancing Translational Sciences