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The Critical Role of IL‐10 in the Antineuroinflammatory and Antioxidative Effects of Rheum tanguticum on Activated Microglia

Year: 2018

Type: article

Abstract: Rheum tanguticum Maxim. ex Balf. (Rt), a traditional Tibetan medicine, is known to exert various bioactivities, including anti‐inflammatory and antioxidative activities. The present study was conducted to investigate anti‐inflammatory and antioxidative effects of Rt on activated microglia. Rt (10 μg/ml) significantly inhibited the mean protein level of interleukin‐1β (IL‐1β) in the organotypic hippocampal slice cultures following treatment with chromogranin A (CGA, 10 nM) and pancreastatin (10 nM), endogenous microglial activators present in senile plaques. Rt also significantly inhibited the expression and production of inflammatory and oxidative molecules, including IL‐1β, tumor necrosis factor‐α, and nitric oxide, by cultured microglia after treatment with CGA. These effects of Rt are considered to be mediated by the secretion of interleukin‐10 (IL‐10) from microglia, because neutralizing antibodies against IL‐10 significantly canceled these effects. To explore the causative components of Rt responsible for inducing the secretion of IL‐10, the effects of seven components of Rt on the IL‐10 expression in microglia were examined. Among them, aloe‐emodin (10 μM) and (+)‐catechin (30 μM) were able to induce the secretion of IL‐10 from cultured microglia. Therefore, aloe‐emodin and (+)‐catechin are deemed responsible for the antineuroinflammatory and antioxidative effects of Rt through the secretion of IL‐10 from microglia. Accordingly, Rt is considered potentially useful for the treatment of AD.

Source: Oxidative Medicine and Cellular Longevity

DOI: 10.1155/2018/1083596