Work

3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells

Year: 2018

Type: article

Abstract: mice were mixed with ID8 cells (TAM:ID8 at a ratio of 1:10) in medium containing 2% Matrigel and seeded onto the 24-well plate precoated with Matrigel. As transcoelomic metastasis is also associated with many other cancers such as pancreatic and colon cancers, TAM-mediated spheroid formation assay would provide a useful approach to define the molecular mechanism and therapeutic targets for ovarian cancer and other transcoelomic metastasis cancers.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.2815

Authors: Lingli Long, Mingzhu Yin, Wang Min

Institutions: Sun Yat-sen University, The First Affiliated Hospital, Sun Yat-sen University, Yale University

Language: English

Topic: Immune cells in cancer

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Heart, Lung, and Blood Institute