Year: 2018
Type: article
Abstract: Today, the need for time-effective and cost-effective nucleic acid detection methods is still growing in fields such as human genotyping and pathogen detection. Using synthetic biomolecular components, many methods have been developed for fast nucleic acid detection 1 , 2 , 3 ; however, they may not be able to satisfy specificity, sensitivity, speed, cost and simplicity at the same time. Recently, a very promising CRISPR-based diagnostic (CRISPR-Dx) (namely SHERLOCK) was established, which was based on the collateral effect of an RNA-guided and RNA-targeting CRISPR effector, Cas13a 4 . SHERLOCK is of high sensitivity and specificity, and is very convenient in detection of target RNA. However, to detect DNA sequences, in vitro transcription of DNA to RNA must be conducted prior to the SHERLOCK test, which could be inconvenient.
Source: Cell Discovery
Authors: Shi-Yuan Li, Qiu-Xiang Cheng, Jing-Man Wang, Xiao-Yan Li, Zi-Long Zhang, Song Gao, Rui-Bing Cao, Guo-Ping Zhao, Jin Wang
Institutions: Chinese Academy of Sciences, Shanghai Institutes for Biological Sciences, Center for Excellence in Molecular Plant Sciences, Shenzhen University, Shenzhen Second People's Hospital, Shanghai Entry-Exit Inspection and Quarantine Bureau, Yangzhou University, Nanjing Agricultural University, Chinese University of Hong Kong, Prince of Wales Hospital
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,590
OpenAlex ID: W2801783434