Work

Lab-on-a-tissue : optimization of on-tissue chemistry for improved mass spectrometry imaging

Year: 2018

Type: dissertation

Abstract: The work presented in this thesis describes the improvement and application of on-tissue chemistry for in-situ biomolecular analysis using matrix assisted-laser desorption/ionization mass spectrometry imaging (MALDI-MSI). We have proposed new methodologies, applying on-tissue (enzymatic) chemistry, to increase the molecular information obtained in a MALDI-MSI analysis. We have also developed an automated histology-guided MSI platform, based on state-of-the-art image processing tools, to facilitate high mass and spatial resolution MALDI-MSI while maintaining reasonable data loads and acquisition times. We have shown the importance of these methods in a clinical biomarker discovery study on myxoid liposarcoma tissues.

Source: Leiden Repository (Leiden University)

Author: Bram Heijs

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: European Commission, ZonMw, National Institutes of Health, National Cancer Institute

Awards: Aberrantly secreted glycoproteins as markers of liver cancer, G7147469967, Methods for high-throughput (HTP) analysis of protein glycosylation, Direct Tumor Glycan Profiling on Tissue Microarrays, Identification of Altered Glycan and Glycoproteins in Viral Induced Liver Cancer

OpenAlex ID: W2801838700

Cites: 302

Cited by:

Related to: 10