A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling
Year: 2018
Type: article
Abstract: Significance ATPases have diverse cellular roles, including extracting proteins from membranes to maintain organellar function. The PEX1–PEX6 heterohexameric ATPases are thought to retrotranslocate PEX5 from the peroxisome membrane, and PEX1–PEX6 dysfunction impairs peroxisome biogenesis in humans and plants. We implemented a pex6 suppressor screen in Arabidopsis and recovered a compensatory pex1 allele that rescues several pex6 defects. Preventing autophagy also improved pex6 peroxisome function, and combining the pex1 and autophagy lesions delivered synergistic benefits. Surprisingly, these different alterations ameliorated pex6 symptoms without notably restoring the sole known function of PEX6, suggesting that PEX1–PEX6 has unexplored functions. Because the pex6 mutations ameliorated by pex1 are analogous to those in human pex6 patients, this study informs research on peroxisome dysfunction in other eukaryotes.
Institutions: Rice University, University of Missouri–St. Louis
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funders: National Science Foundation, Welch Foundation, National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, National Center for Research Resources, National Center for Advancing Translational Sciences, Office of Extramural Research, National Institutes of Health
Awards: G1064601076, G3561346444, Chemical and genetic modifiers of peroxisome function in Arabidopsis, Support Framework for Excellence in Interdisciplinary Research and Education, Washington University Institute of Clinical and Translational Sciences, Laser scanning confocal microscope, Washington University Institute of Clinical and Translational Sciences (UL1), Graduate Research Fellowship Program, Peroxisome dynamics - biogenesis and turnover, Washington University Institute of Clinical and Translational Sciences, Cancer Center Support Grant
OpenAlex ID: W2802006538