Work

Protein Synthesis Initiation in Eukaryotic Cells

Year: 2018

Type: preprint

Abstract: G cap-dependent mechanism, which is a highly coordinated and stepwise regulated process that requires the combined action of at least 12 distinct translation factors with initiator transfer RNA (tRNA), ribosomes, and mRNAs. We limit our review to studies involving either mammalian or budding yeast cells and factors, as these represent the two best-studied experimental systems, and only include a reference to other organisms where particular insight has been gained. We close with a brief description of what we feel are some of the major unknowns in eukaryotic initiation.

Source: Cold Spring Harbor Perspectives in Biology

DOI: 10.1101/cshperspect.a033092

Authors: William C. Merrick, Graham D. Pavitt

Institutions: Case Western Reserve University, Manchester Academic Health Science Centre, University of Manchester

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Biotechnology and Biological Sciences Research Council

Awards: Specialised ribosomes facilitating cellular responses to oxidative stress, Investigating novel steps for promoting tRNA binding to translation factor eIF2 during protein synthesis initiation, Structural studies of eukaryotic protein synthesis factor complexes eIF2B and eIF2/eIF2B, critical for translational control in eukaryotic cells, GTP-binding to eIF2B as a novel mechanism for G protein activation in protein synthesis initiation