Systematic mapping of contact sites reveals tethers and a function for the peroxisome-mitochondria contact
Year: 2018
Type: article
Abstract: The understanding that organelles are not floating in the cytosol, but rather held in an organized yet dynamic interplay through membrane contact sites, is altering the way we grasp cell biological phenomena. However, we still have not identified the entire repertoire of contact sites, their tethering molecules and functions. To systematically characterize contact sites and their tethering molecules here we employ a proximity detection method based on split fluorophores and discover four potential new yeast contact sites. We then focus on a little-studied yet highly disease-relevant contact, the Peroxisome-Mitochondria (PerMit) proximity, and uncover and characterize two tether proteins: Fzo1 and Pex34. We genetically expand the PerMit contact site and demonstrate a physiological function in β-oxidation of fatty acids. Our work showcases how systematic analysis of contact site machinery and functions can deepen our understanding of these structures in health and disease.
Source: Nature Communications
Authors: Nadav Shai, Eden Yifrach, Carlo W. T. van Roermund, Nir Cohen, Chen Bibi, Lodewijk IJlst, Laetitia Cavellini, Julie Meurisse, Ramona Schuster, Lior Zada, Muriel C. Mari, Fulvio M. Reggiori, Adam L. Hughes, Mafalda Escobar-Henriques, Mickael M. Cohen, Hans R. Waterham, Ronald J. A. Wanders, Maya Schuldiner, Einat Zalckvar
Institutions: Weizmann Institute of Science, Amsterdam UMC Location University of Amsterdam, University of Amsterdam, Centre National de la Recherche Scientifique, Sorbonne Université, Institut de Biologie Physico-Chimique, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Université Pierre-et-Marie-Curie, University of Cologne, Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases, University Medical Center Groningen, University of Groningen, University of Utah
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Awards: G3055860283, G3182549475, G3748436048, G464257056, G4706951580, G4971532933, G664478000, G7420245167, G8062106642, G825885378, Dynamique des membranes transductrices d'énergie : biogénèse et organisation supramoléculaire., Structural dissection of mitochondrial tethering through super-resolution imaging, G9000063464, G9000244658
OpenAlex ID: W2802226968