Work

Cardiomyocyte Maturation Requires TLR3 Activated Nuclear Factor Kappa B

Year: 2018

Type: article

Abstract: The process by which committed precursors mature into cardiomyocytes is poorly understood. We found that TLR3 inhibition blocked cardiomyocyte maturation; precursor cells committed to the cardiomyocyte lineage failed to express maturation genes and sarcomeres did not develop. Using various approaches, we found that the effects of TLR3 upon cardiomyocyte maturation were dependent upon the RelA subunit of nuclear factor kappa B (NFκB). Importantly, under conditions that promote the development of mature cardiomyocytes NFκB became significantly enriched at the promoters of cardiomyocyte maturation genes. Furthermore, activation of the TLR3-NFκB pathway enhanced cardiomyocyte maturation. This study, therefore, demonstrates that the TLR3-NFκB pathway is necessary for the maturation of committed precursors into mature cardiomyocytes. Stem Cells 2018;36:1198-1209.

Source: Stem Cells

DOI: 10.1002/stem.2833

Authors: Conrad P. Hodgkinson, Richard E. Pratt, Imke Kirste, Sophie Dal-Pra, John P. Cooke, Victor J. Dzau

Institutions: Duke University, Duke Medical Center, Houston Methodist Research Institute

Language: English

Topic: Congenital heart defects research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Edna and Fred L. Mandel, Jr. Foundation, National Heart, Lung, and Blood Institute