NUFIP1 is a ribosome receptor for starvation-induced ribophagy
Year: 2018
Type: article
Abstract: The lysosome degrades and recycles macromolecules, signals to the master growth regulator mTORC1 [mechanistic target of rapamycin (mTOR) complex 1], and is associated with human disease. We performed quantitative proteomic analyses of rapidly isolated lysosomes and found that nutrient levels and mTOR dynamically modulate the lysosomal proteome. Upon mTORC1 inhibition, NUFIP1 (nuclear fragile X mental retardation-interacting protein 1) redistributes from the nucleus to autophagosomes and lysosomes. Upon these conditions, NUFIP1 interacts with ribosomes and delivers them to autophagosomes by directly binding to microtubule-associated proteins 1A/1B light chain 3B (LC3B). The starvation-induced degradation of ribosomes via autophagy (ribophagy) depends on the capacity of NUFIP1 to bind LC3B and promotes cell survival. We propose that NUFIP1 is a receptor for the selective autophagy of ribosomes.
Source: Science
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
Awards: Regulation of the mTOR Pathway by Nutrients, Novel Components of the mTORC1 and mTORC2 Pathways, Cell Growth Signaling in Cancer Development, G639478639, G647031170, ROLE OF LYSOSOMAL TRANSPORTERS IN PROMOTING THE GROWTH OF CLEAR CELL RENAL CELL CARCINOMA AND OTHER TUMOR TYPES, Pre-Doctoral Training in Biological Sciences, G8759813349
OpenAlex ID: W2802593879