Work

Self-replication of DNA by its encoded proteins in liposome-based synthetic cells

Year: 2018

Type: article

Abstract: Replication of DNA-encoded information and its conversion into functional proteins are universal properties of life. In an effort toward the construction of a synthetic minimal cell, we implement here the DNA replication machinery of the Φ29 virus in a cell-free gene expression system. Amplification of a linear DNA template by self-encoded, de novo synthesized Φ29 proteins is demonstrated. Complete information transfer is confirmed as the copied DNA can serve as a functional template for gene expression, which can be seen as an autocatalytic DNA replication cycle. These results show how the central dogma of molecular biology can be reconstituted and form a cycle in vitro. Finally, coupled DNA replication and gene expression is compartmentalized inside phospholipid vesicles providing the chassis for evolving functions in a prospective synthetic cell relying on the extant biology.

Source: Nature Communications

DOI: 10.1038/s41467-018-03926-1

Authors: Pauline van Nies, Ilja Westerlaken, Duco Blanken, Margarita Salas, Mario Mencía, Christophe Danelon

Institutions: Delft University of Technology, Consejo Superior de Investigaciones Científicas, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid

Language: English

Topic: Origins and Evolution of Life

Subfield: Astronomy and Astrophysics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $6,990

Funders: Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Ministerio de Economía y Competitividad