New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi
Year: 2018
Type: article
Abstract: CRISPR-mediated genome engineering technologies have revolutionized genetic studies in a wide range of organisms. Here we describe new vectors and guide sequences for CRISPR mutagenesis in the important human fungal pathogens C. albicans and C. glabrata , as well as in the related yeasts S. cerevisiae and N. castellii . The design of these vectors enables efficient serial mutagenesis in each of these species by leaving few, if any, exogenous sequences in the genome. In addition, we describe strategies for the creation of unmarked deletions in each of these species and vector designs that permit the creation of vector libraries for pooled screens. These tools and strategies promise to advance genetic engineering of these medically and industrially important species.
Source: mSphere
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,500
Awards: Graduate Research Fellowship Program, Roles for mRNA methylation and lncRNA in Candida mating and pathogenicity, Pre-Doctoral Training in Biological Sciences, Genetic Control of Nutrition Starvation in Yeast, G9000175394, G9000314117, Distinguishing features of C. albicans pseudouridylation and their roles in pathogenesis, Post-transcriptional gene regulation
OpenAlex ID: W2802860499