Late-stage deuteration of 13 C-enriched substrates for T 1 prolongation in hyperpolarized 13 C MRI
Year: 2018
Type: article
Abstract: A robust and selective late-stage deuteration methodology was applied to 13C-enriched amino and alpha hydroxy acids to increase spin-lattice relaxation constant T1 for hyperpolarized 13C magnetic resonance imaging. For the five substrates with 13C-labeling on the C1-position ([1-13C]alanine, [1-13C]serine, [1-13C]lactate, [1-13C]glycine, and [1-13C]valine), significant increase of their T1 was observed at 3 T with deuterium labeling (+26%, 22%, +16%, +25% and +29%, respectively). Remarkably, in the case of [2-13C]alanine, [2-13C]serine and [2-13C]lactate, deuterium labeling led to a greater than four fold increase in T1. [1-13C,2-2H]alanine, produced using this method, was applied to in vitro enzyme assays with alanine aminotransferase, demonstrating a kinetic isotope effect.
Source: Chemical Communications
DOI: 10.1039/c8cc02246a
Institution: University of California, San Francisco
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: G1600419044, G1852526301, G2112503047, Clinical translation of D-amino acid derived PET tracers for imaging spinalinfection, G2683385928, G3187569508, G3299962541, Ascorbate-based biomarkers for predicting radiation response in prostate cancer, G3881180584, G4423664179, G6100379443, Hyperpolarized MRI Technology Resource Center
OpenAlex ID: W2802887566