Hydrogel-Tissue Chemistry: Principles and Applications
Year: 2018
Type: review
Abstract: Over the past five years, a rapidly developing experimental approach has enabled high-resolution and high-content information retrieval from intact multicellular animal (metazoan) systems. New chemical and physical forms are created in the hydrogel-tissue chemistry process, and the retention and retrieval of crucial phenotypic information regarding constituent cells and molecules (and their joint interrelationships) are thereby enabled. For example, rich data sets defining both single-cell-resolution gene expression and single-cell-resolution activity during behavior can now be collected while still preserving information on three-dimensional positioning and/or brain-wide wiring of those very same neurons-even within vertebrate brains. This new approach and its variants, as applied to neuroscience, are beginning to illuminate the fundamental cellular and chemical representations of sensation, cognition, and action. More generally, reimagining metazoans as metareactants-or positionally defined three-dimensional graphs of constituent chemicals made available for ongoing functionalization, transformation, and readout-is stimulating innovation across biology and medicine.
Source: Annual Review of Biophysics
Authors: Viviana Gradinaru, Jennifer Treweek, Kristin Overton, Karl Deisseroth
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: green
Awards: Intact Circuit Assessment of Aging Dopamine Neurons vis Optogenetics and CLARITY, Neuromodulation and Neurodegeneration: the Missing Link and Mechanisms of Action, Comprehensive Structural and Functional Mapping of the Mammalian Cardiac Nervous System, Technology development for fast optical interrogation of living neural circuitry, Structural and molecular identification of circuitry underlying joint processing of motivation and aversion, Neural circuit dynamics of drug action:revealing, uncoupling, and restoring altered brain states, Brain-spanning and scale-crossing circuitry mediating drive function and dysfunction
OpenAlex ID: W2803335887