Nutrient Requirements for in vitro Propagation of Ricinus communis L. Mature Zygotic Embryos
Year: 2017
Type: article
Abstract: Background and Objective: Ricinus communis (R. communis) is famous for its economic values and of great importance to researchers interested in the raising plantlets from hybrids.This study was carried out on the nutrient requirements for the in vitro propagation of R. communis employing three basal media using zygotic embryos as explants.Materials and Methods: Following the maintenance of asepsis, zygotic embryos were excised from mature seeds and cultured on the three basal media with 3% sucrose and 8 g LG 1 of agar.Plant growth regulators were not added to the media.This study was done to determine the most suitable basal medium for the growth of R. communis zygotic embryo.Results: The results obtained showed that the three basal media employed supported in vitro regeneration of the embryo explants.The highest mean shoot length (4.450±0.231cm), the highest mean root length (2.190±0.262cm), highest mean fresh weight (0.365±0.032 g), highest mean leaf area (1.999±0.189cm 2 ), highest mean percent sprouting (91.660±0.000)and highest mean number of roots (4.600±0.163)were observed on Murashige and Skoog medium whereas the highest mean sprout rate (0.330±0.000) was obtained on Murashige and Skoog and Gamborg B5 media.The embryo explants were able to develop into normal plantlets even in the absence of growth regulators.This may determine that endogenous hormones in the zygotic embryos were present at an optimal level to support regeneration.Conclusion: Results from this study indicated that Murashige and Skoog basal medium was the best basal medium for the in vitro propagation of R. communis zygotic embryos and for mass production.
Authors: G.C. Louis, C.U. Okafor, C.E.A. Okezie
Institution: University of Nigeria
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2803543948