S-MiRAGE: A Quantitative, Secreted RNA-Based Reporter of Gene Expression and Cell Persistence
Year: 2018
Type: article
Abstract: Nondestructive measurements of cell persistence and gene expression are crucial for longitudinal research studies and for prognostic assessment of cell therapies. Here we describe S-MiRAGE, a platform that utilizes small secreted RNA molecules as sensitive and quantitatively accurate reporters of cellular processes. S-MiRAGE allows cellular numbers or gene expression to be measured from culture media or from biofluids. We show that multiple S-MiRAGE reporters can be multiplexed, and demonstrate the utility of S-MiRAGE by monitoring the differentiation status of human embryonic stem cells in vitro and tumor growth in a mouse model in vivo.
Source: ACS Synthetic Biology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: National Science Foundation, U.S. Department of Defense, Cancer Prevention and Research Institute of Texas, Ladies Leukemia League, Universidad de Puerto Rico, National Institutes of Health, University of Texas MD Anderson Cancer Center, National Cancer Institute, Division of Molecular and Cellular Biosciences, Office of Strategic Coordination, National Center for Advancing Translational Sciences, Common Fund
Awards: G1102459868, G1573574303, G1852139970, G2226474896, MD Anderson Cancer Center Prostate Cancer SPORE, G2663453512, G3190990280, Recruitment of First-Time, Tenure-Track Faculty Members, Protein-coding and non-coding RNA biomarkers for early detection of CLL, G5306837625, CAREER: Understanding human embryonic development from the bottom up, G6077865511, G8090489057, G9000065446, G9000339540
OpenAlex ID: W2803732849