Genetic and Imaging Approaches Reveal Pro-Inflammatory and Immunoregulatory Roles of Mast Cells in Contact Hypersensitivity
Year: 2018
Type: article
Abstract: Contact hypersensitivity (CHS) is a common T cell-mediated skin disease induced by epicutaneous sensitization to haptens. Mast cells (MCs) are widely deployed in the skin and can be activated during CHS responses to secrete diverse products, including some with pro-inflammatory and anti-inflammatory functions. Conflicting results have been obtained regarding pathogenic versus protective roles of MCs in CHS, and this has been attributed in part to the limitations of certain models for studying MC functions in vivo. This review discusses recent advances in the development and analysis of mouse models to investigate the roles of MCs and MC-associated products in vivo. Notably, fluorescent avidin-based two-photon imaging approaches enable in vivo selective labeling and simultaneous tracking of MC secretory granules (e.g., during MC degranulation) and MC gene activation by real-time longitudinal intravital microscopy in living mice. The combination of such genetic and imaging tools has shed new light on the controversial role played by MCs in mouse models of CHS. On the one hand, they can amplify CHS responses of mild severity while, on the other hand, can limit the inflammation and tissue injury associated with more severe or chronic models, in part by representing an initial source of the anti-inflammatory cytokine IL-10.
Source: Frontiers in Immunology
Language: English
Topic: Mast cells and histamine
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
Funders: Tobacco-Related Disease Research Program, Fondation Acteria, European Commission, Institut National de la Santé et de la Recherche Médicale, United States-Israel Binational Science Foundation, Fonds De La Recherche Scientifique - FNRS, Fondation pour la Recherche Médicale, Société Française de Dermatologie et de Pathologie Sexuellement Transmissible, Walloon excellence in life sciences and biotechnology, National Institutes of Health, Horizon 2020 Framework Programme, Sean N. Parker Center for Allergy and Asthma Research, Stanford University, National Institute of Allergy and Infectious Diseases, National Institute of Arthritis and Musculoskeletal and Skin Diseases
Awards: G1121861823, G1134578003, G2025007194, G2596785601, G270099223, G3291143030, G3345895242, G444668770, G4937468798, G504025110, G526393511, Neuron/mast cell interactions in skin diseases, G5854089270, Blocking peanut allergy through protective IgG antibodies, G5954123636, G6329715174, Role of nociceptive sensory neuron/mast cell interactions in cutaneous allergic inflammation, G8130309616, G8489695953, G8512683158, G8858395165, G9000095744, G9000187821, Integrated Genomic and Functional Studies of Immunotherapy for Multi-Food Allergy
OpenAlex ID: W2803906736