Year: 2019
Type: article
Abstract: The CRISPR-Cas9 system provides the ability to edit, repress, activate, or mark any gene (or DNA element) by pairing of a programmable single guide RNA (sgRNA) with a complementary sequence on the DNA target. Here we present a new method for small-molecule control of CRISPR-Cas9 function through insertion of RNA aptamers into the sgRNA. We show that CRISPR-Cas9-based gene repression (CRISPRi) can be either activated or deactivated in a dose-dependent fashion over a >10-fold dynamic range in response to two different small-molecule ligands. Since our system acts directly on each target-specific sgRNA, it enables new applications that require differential and opposing temporal control of multiple genes.
Source: Nature Communications
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,200
Funders: National Science Foundation, Howard Hughes Medical Institute, U.S. Department of Health and Human Services, W. M. Keck Foundation, National Institutes of Health, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering
Awards: G1064193810, G1524328802, Molecular Biophysics Training Grant, G2275164664, G3892861298, G4932001829, G5013145805, Engineering CRISPR-Cas proteins for conditional and robust interrogation of the genome, Computational design of Cas9-based molecular imaging reagents, G6204560716, G7004123550, G738160294, Mechanism and therapeutic application of RNA-guided immune systems, Computational design of new protein structures and interactions
OpenAlex ID: W2953209503