Year: 2016
Type: article
Abstract: BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) is a wellknown pathogen that causes serious diseases in humans.As part of the efforts to control this pathogen, an isolated bacteriophage, Siphoviridae, which specifically targets Methicillin-resistant Staphylococcus aureus (MRSA), was characterized. AimsThe objective of this study was to characterize of a virulent bacteriophage (Siphoviridae) isolated from a NICU bathroom sink. MethodsThe MRSA strain was isolated from patient blood.The isolated strain was confirmed as MRSA using conventional methods.Phages were isolated from a NICU bathroom sink and activity was lytic as determined by spot test.Titer phage lysate was measured by the Double Layer Agar (DLA) technique.The morphology was found with electron microscopy.The single-step growth curve was plotted. ResultsElectron microscopy showed the phage as a member of the family Siphoviridae, serogroup A and F. The isolated phage was capable of lytic activity against methicillin-resistant Staphylococcus aureus (MRSA) strain as shown by spot test.By DLA, the titre of the phages was determined to be 10×10 8 PFU/ml.The single-step growth curve showed that the latent period of the isolated bacteriophage was 30 min and the total number of viable progeny per infected host, burst size, was 2600 PFU/infected host. ConclusionIn this study, two phages were isolated and characterized from a NICU bathroom sink, from the Siphoviridae family, which specifically targets methicillin-resistant Staphylococcus aureus (MRSA).
Source: Australasian Medical Journal
Institution: Mazandaran University of Medical Sciences
Language: English
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2467686372