Work

The Mode of Incorporation of 6-Benzylaminopurine into Tobacco Callus Transfer Ribonucleic Acid

Year: 1974

Type: article

Abstract: Cytokinin-dependent tobacco (Nicotiana tabacum) tissue was grown in the presence of N(6)-benzyladenine (6-benzylaminopurine) labeled in the phenyl ring with (3)H and in the 8-position of the purine ring with (14)C. The (3)H/(14)C ratio of N(6)-benzyladenosine recovered from labeled tobacco callus transfer RNA preparations was compared with the corresponding ratio for N(6)-benzyladenine supplied in the medium. By this means, with suitable controls, the incorporation of the synthetic cytokinin, N(6)-benzyladenine, into the transfer RNA of tobacco callus tissue was shown to involve the intact moiety. The observed level of incorporation was up to one N(6)-benzyladenosine molecule per 10(4) transfer RNA molecules.

Source: PLANT PHYSIOLOGY

DOI: 10.1104/pp.54.5.737

Authors: Graham C. Walker, Nelson J. Leonard, Donald J. Armstrong, Norimoto Murai, Folke Skoog

Institutions: University of Illinois Urbana-Champaign, University of Illinois System, University of Wisconsin–Madison

Language: English

Topic: Plant Molecular Biology Research

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, Wisconsin Alumni Research Foundation, National Institutes of Health, University of Illinois at Urbana-Champaign

OpenAlex ID: W1992636412